FacsCanto II, BD Biosciences

This cytometer is equipped with 3 lasers:

OOB BioPIC analyseur
  • A blue Argon one 488nm, whose optical orthogonal pathway allow the assessment and separation of fluorescence signals thanks to 4 detectors: FL1: 530/30nm, FL2: 585/42nm, FL3: 670 LP, FL4: 780/60nm.
  • A red one 633nm: FL5 BP 750/60nm, FL6 BP 650/20nm
  • A violet one 405nm: FL7 BP 502-535nm, FL8 BP 425-475nm

Diva6 is commonly used to work on the datas under Windows XP operating system.

Cytoflex, Beckman Coulter

This cytometer is equipped with 2 lasers:

OOB BioPIC analyseur
  • A blue one 488nm, whose optical pathway allow the assessment and separation of fluorescence signals thanks to 3 detectors (APD): FITC 525/40nm, PE 585/42nm, PC5.5 690/50nm. Two scattering detectors linked to the 488 source: SSC and FSC.
  • A red one 638nm: APC 660/20nm, APC A750 780/60nm.

CytExpert is commonly used to work on the datas under Windows 7 operating system.

Instruments are available through GRR reservation (lab intranet).

Oceanographic application:

Flow cytometry allows a good understanding of the complex structure of phytoplankton community. Phytoplankton community is mainly composed of unicellular algae ‹3µm. This cytometric technique enable the seasonal monitoring of the phytoplankton evolution in aquatic ecosystems. This single-cell cellular approach of the ecosystems led to new shade on oceanographic functioning, specifically in biogeochemical studies of the ocean.

New, very small and very abundant microalgae species have been discovered through flow cytometry analyses:Prochlorococcus sp. (Chisholm et al. 1988) or Ostreococcus tauri (Courties et al. 1994). Their primordial role in the trophic food web functioning is mainly studied through flow cytometer & cell sorter, sometimes shipped on marine vessel.

Here you have an example of cytogram that show phytoplankton populations where each one of the cells are identified by scatters & fluorescences.

OOB BioPIC peuplement phytoplanctonique

Cell sorter

FacsAria I, BD Biosciences

OOB BioPIC trieur de cellules

This cell sorter included:

  • Two lasers : 488nm & 633nm
  • 8 fluorescences & 2 scatters
  • Quartz flow-cell (high sensitivity)
  • Sorting by electrostatic deflection in the air
  • 4 ways sorting

Marine bacteriology application:

We use green dye Syber Green I to count marine bacteria (millions/mL). These bacteria use to show 2 or 3 populations weakly fluorescent (LNA) or highly fluorescent (HNA) and this fluorescence is linked to their activity.

Bacteria cell sorting, identified through green fluorescence and scatter, associated to specific metabolisms analyses or characterized in molecular biology, lead to specify these activities and their diversity still mainly unknown in aquatic ecosystems.

Solid phase laser scanning cytometer

Solid phase laser scanning cytometer, coupled with an inverted fluorescence microscope, is an essential tool to detect and quantify, thanks to specific fluorochromes, pathogenics bacteria or toxic algae in aquatic ecosystems.

Observatoire Océanologique
de Banyuls-sur-Mer
1 avenue Pierre Fabre
66650 Banyuls-sur-Mer
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