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Article 4

AQUAPHILUS DOLOMIAE GEN. NOV., SP. NOV., ISOLATED FROM A DEEP AQUIFER

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M. Bourrain*1,2,3, C. Villette1,2,3, T. Nguyen4, P. Lebaron1,2
1 UPMC Univ Paris 06, UMR 7621, LOMIC, Observatoire Océanologique, F-66650, Banyuls/Mer, France
2 CNRS, UMR 7621, LOMIC, Observatoire Océanologique, F-66650, Banyuls/Mer, France
3 Pierre Fabre Dermocosmétique, F-31035 Toulouse, France
4 Institut de Recherche Pierre Fabre, F-31035 Toulouse, France
Corresponding author: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

ABSTRACT. – A novel chemoorganotrophic, non spore forming bacterium with a cell size of 2.3 ± 0.3 μm long and 1.0 ± 0.1 μm wide was isolated from aquifer water in the Cévennes mountains, France. Cells of the isolate, designated strain LMB64T, were motile, straight rods with a single polar flagellum, and are able to accumulate poly-β-hydroxybutyrate granules. Growth was observed at temperatures between 14 and 45°C (optimum 30°C) and pH between 6·0 and 8·0 (optimum pH = 6·0). NaCl was not required for growth. Catalase, oxidase and arginine dihydrolase activities and indole production were detected. The major cellular fatty acids were C16:0 and C16:1 ω7c or/and C16:1 ω6c (40.1 and 36.2 %, respectively). The predominant respiratory quinone was ubiquinone 8 (Q-8), but another dominant compound, which did not elute with Q8 or any documented quinone, was observed. The G+C content of the genomic DNA was 64.2 mol %. A phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain LMB64T forms a distinct lineage within the family Neisseriaceae of the β-Proteobacteria class. The strain LMB64T appears closely related to Pseudogulbenkiania subflavaT (95.7 % 16S rRNA gene sequence similarity), Gulbenkiania mobilisT (94.7 %) and Paludibacterium yongneupenseT (94.6 %). On the basis of its physiological and molecular properties, the strain LMB64T (= CNCM I-4290) is proposed as the type strain of Aquaphilus dolomiae gen. nov., sp. nov.

You are here: Volume 62 (2012) Issue 4 Article 4
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